Home
HelpTest Code ADM13 ADAMTS13 Activity and Inhibitor Profile, Plasma
Reporting Name
ADAMTS13 Activity and Inhibitor ProfileUseful For
Assisting with the diagnosis of congenital or acquired thrombotic thrombocytopenic purpura
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADMFX | ADAMTS13 Activity Assay | No | Yes |
| ADMIN | ADAMTS13 Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADMIS | ADAMTS13 Inhibitor Screen | No | No |
| ADMBU | ADAMTS13 Inhibitor Bethesda Titer | No | No |
Testing Algorithm
Testing begins with the ADAMTS-13 activity assay to evaluate the percent activity. If the ADAMTS-13 activity is less than 30%, an inhibitor screen will be performed to look for specific ADAMTS-13 inhibition. If specific inhibition is apparent, the titer of the inhibitor will be determined.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma Na CitShipping Instructions
Send both vials in the same shipping container.
Specimen Required
Patient Preparation: Fasting preferred
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vials
Specimen Volume: 2 mL in 2 plastic vials each containing 1 mL
Collection Instructions:
1. Specimen must be collected prior to replacement therapy.
2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing
3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
4. Aliquot plasma (1 mL per aliquot) into 2 separate plastic vials, leaving 0.25 mL in the bottom of centrifuged vial.
5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, below -40° C.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. If priority specimen, mark request form, give reason, and request a call-back.
3. Each coagulation assay requested should have its own vial.
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Special Instructions
Reference Values
ADAMTS13 ACTIVITY ASSAY
≥70%
ADAMTS13 INHIBITOR SCREEN
Negative
ADAMTS13 BETHESDA TITER
<0.4 BU
Day(s) Performed
Monday through Friday, Sunday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
85397
85335 (if appropriate)
85335 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ADM13 | ADAMTS13 Activity and Inhibitor Profile | 53622-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 61211 | ADAMTS13 Activity Assay | 53622-7 |
| 34586 | ADAMTS13 Interpretation | 69049-5 |
Report Available
1 to 3 daysReject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
Fluorescence Resonance Energy Transfer (FRET)
Forms
1. Coagulation Patient Information (T675)
2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Coagulation Test Request (T753)
-Renal Diagnostics Test Request (T830)